Cell Bio III (recitation vocab)

29 cards·by Simiankolya

Genetic alteration of a cell from the direct uptake of exogenous genetic material through a cell's membrane

Transformation

Transformation is genetic _ of a cell from the direct uptake of _ genetic material through a cell's _

Alteration, exogenous, membrane

A gene that researchers attach to a regulatory sequence of another gene of interest, thatconveys an identifiable phenotype

Reporter gene

A reporter gene is a gene that researchers attach to a _ sequence of another gene of interest, that conveys an identifiable _

Regulatory, phenotype

A technique used to isolate an individual gene using conditional selection on bacteria

Complementation assay

A protein or factor that affects expression of genes and their transcription

Transcriptional regulator

A segment of a nucleic acid molecule which is capable of increasing or decreasing theexpression of specific genes within an organism

Regulatory sequence

A regulatory sequene is a segment of a _ molecule which is capable of increasing or decreasing the _ of specific genes within an organism

Nucleic acid, expression

A mutation that has a wild-type phenotype under certain conditions, and a mutant phenotype under other condtions

Conditional mutation

A mutation that has a wild-type phenotype under certain conditions, and a mutant phenotype under other condtions

Conditional mutation

The use of a laser to remporarily disrupt protein activity on the membrane of a cell's membrane

Photobleaching

Photobleaching is the use of a _ to remporarily disrupt protein activity on the _ of a cell

Laser, membrane

Inferring the activity and dynamics of membrane proteins in a cell based on how readily they recover after bleached with a laser

FRAP

FRAP stands for _

Fluorescence Recovery After Photobleaching

GFP stands for _

Green Fluorescent Protein

A substance that evokes the production of more antibodies

Antigen

An antigen is a substance that evokes the production of more _

Antibodies

The part of an antigen that is recognized by the immune system, specifically by antibodies, B cells, or T cells

Epitope

The epitope is the part of an _ that is recognized by the immune system, specifically by antibodies, _ cells, or _ cells

Antigen, B, T

The part of an antibody which recognizes an antigen, the antigen-binding site of an antibody

Paratope

The paratope is the part of an _ which recognizes an antigen, the _-binding site of an antibody

Antibody, antigen

Monospecific antibodies that are the same because they are made by identical immune cells that are all clones of a unique parent cell

Monoclonal antibodies

Monoclonal antibodies are _ antibodies that are the same because they are made by identical _ cells that are all clones of a unique _

Monospecific, immune, parent cell

Antibodies that are obtained from different B cells, and each one identifies a different epitope on the same antigen

Polyclonal antibodies

Polyclonal antibodies are obtained from different _, and each one identifies a different _ on the same _

B cells, epitope, antigen

A structural gene in the Lac operon that encodes beta-galactosidase, which cleaves lactose into glucose and galactose

LacZ

LacZ is a _ gene in the Lac _ that encodes beta-_, which cleaves lactose into _ and galactose

Structural, operon, galactosidase, glucose

An organic compound consisting of galactose linked to a substituted indole, used in molecular biology to test for beta-galactosidase

X-gal

X-gal is an organic compound consisting of _ linked to a substituted _, used in molecular biology to test for beta-_

Galactose, indole, galactosidase