GenPath MCGavin

chapter 1

28 cards·by santhipriyadarsini

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lesion could resolve or become worse due to unforseen factors:

PAT: guarded

lesion expected to resolve with no lasting harm:

PAT good or excellent

If animal is not expected to recover from the lesion or dz:

PAT: grave

Accurate determination of prognosis demands a thorough understanding of:

PAT

Hematoxylin stains blue

nucleus, ribosomes and mitochondria

Eosin stain red

intra and extracellular proteins

intracellular proteins stained by eosin

enzymes, actin, myosin

Extracellular proteins stained by Eosin

collagen and ECM

specific antigens are identified in tisues by antibodies linked to a chromogen

IHC

labeled NA probes can identify complementary strands of host or microbe DNA or RNA in intact cells or tissues

ISH

Target DNA of microbes in small amounts in biological material can be amplified and identified

PCR

which one is highly sensititve IHC, PCR, ISH

PCR

To visualize subcellular structures of cells and microbes

resolution of ultrafine structures of less than a nm

TEM (Transmission electron mcroscopy)

ultrafine observation of surfaces

Scanning EM (SEM)

to get the genetic fingerprint of a disease process

DNA microarray on Lasercapture microdissection samples

To isolate a group of similar tumor cells or a diseased tissue

Lasercapture microdissection (LMD)

emphasized the importance of organs and tissues as the seat of dz

Morgagni and Bichat

Individual cells are primary cause of abnormal function and structure assoc w dzs

Virchow

cytocavitary network

interconnecting cellular system of membrane bound spaces

type and quantity of specific enzymes assoc w each membrane and its cytoplasmic matrix determines

the function of the organelles

targets for injury by microbes, harmful envir agents, genetic, metabolic, toxicological dzs

cell membranes and organelles

surrounds the nucleus

nuclear membrane

organelles within the cell

free ribosomes, ER bound ribosomes (RER), SER, Golgi apparatus and vesicles, mitochondria, lysosome, peroxisome, centrosomes, centrioles

within the nucleus

nucleolus

-forms boundaries of many organelles abd seperate them from the cytosol

cell membrane

phospho lipid bilayer impermeable to watersoluble compounds but permeable to may specific proteins

cell membrane

2 basic /biological functions of cell membrane

1. selective barrier 2. form structual base for enzymes and receptors that determine the cell function