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chapter 7

88 cards·by Alaynab624
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What does being an Aerobe mean?
Utilizes oxygen and can detoxify it
What does being an obligate aerobe mean?
Cannot grow without oxygen
what does being a Facultative Anaerobe mean?
utilizes oxygen but can also grow in its absense.
What does being Microaerophilic mean?
Requires only a small amount of oxygen
What does being anaerobe mean?
Does not utilize oxygen
what does being obligate anaerobe mean?
Lacks the enzyme to detoxify oxygen so it cannot survive in an oxygen environment.
What does Aerotolerant Anaerobes mean?
Do not utilize oxygen but can survive and grow in its presence.
Most cells have developed enzymes to neutralize what chemicals?
Superoxide, dismutase, and catalase
What happens when a microbe is not capable of dealing with toxic oxygen?
It is forced to live in oxygen free habitats
As oxygen is utilized it is transformed into what several toxic products?
Singlet oxygen, Superoxide ion, peroxide, and hydroxyl radicals.
Obligate aerobes need what?
molecular oxygen (O2)
Microaerophilic have O2 levels between...
2 and 10%. ex: Heliobacter pylori
What aerobic organism can use anaerobic respiration or fermentation?
Facultative Anaerobe ex: E. coli, Bacillus anthracis
what anaerobe can have some O2 in environment and has some of the enzymes that rid of the bad oxidizers, Lactobacillus
Aerotolerant Anaerobe
What cannot use O2 and cannot tolerate it in the environment?
Strict Anaerobe
Large rod-shaped, Gram positive, facultatively anaerobic, usually lives in soil, forms endospores
Bacillus anthracis
Pathogenic forms-plasmid with code for anthrax toxins. Ingestion-not so serious. Cutaneous- eschars, can produce toxemia.
Bacillus anthracis
What causes toxemia?
Bacillus anthracis
What toxin prevents muscular contractions?
Clostirdium botulinum
Gram positive, bacilus, anaerobic, endospore former
Clostridium botulinum
Is Bacillus anthracis gram positive or gram negative?
Gram positive
Is bacillus anthracis aerobic, anaerobic, or facultatively anaerobic?
Facultatively anaerobic
What causes tetanus?
Clostridium tetani
Is Clostridium gram positive or negative?
Gram positive
Is Clostridium obligate anaerobe or aerobic?
Obligate Anaerobe
Gram positive bacillus, obligate anaerobe, endospore-former. Causes tetanus
Clostridium tetani
Capnophile..
Grows best at higher Co2 tensions than normally present in the atmosophere
What grows better at higher CO2 tensions than normally present in the atmosphere
Capnophile
All microbes require what in their metabolism?
Carbon Dioxide
Neutrophils..
Grown at a pH between 6 and 8
What microorganisms grow at a pH of 6-8?
Neutrophiles
What grows at extreme acid pH?
Acidophiles
What grows at extreme alkaline pH?
Alkalinophiles
Alkalinophiles live in soils and waters..true or false
True
What is the only human pathogen that can live at pH greater than 8 (funny shaped cell and has 2 BIG chromosomes
Vibrio cholerae
Vibrio cholerae is a Acidophile or a Alkalinophile?
Alkalinophile
What was the first thing Barry Marshall do to apply Koch's Postulates to demonstrate that Heliobacter pylori cause ulcers?
Bacteria invade mucous and attach to gastric epithelial cells.
What was the second thing Barry Marshall do to apply Koch's Postulates to demonstrate that Heliobacter pylori cause ulcers?
Heliobacter, its toxins, and inflammation cause the layer of mucus to become thin
What was the third thing Barry Marshall do to apply Koch's Postulates to demonstrate that Heliobacter pylori cause ulcers?
Gastic acid detroys epithelial cells and underlying tissue.
Most microbes exist under what conditions
Hypotonic or isotonic
What require a high concentration of salt?
Halophiles
What does not require high concentrations of solute but can tolerate it when it occurs?
Osmotolerant
What is Isotonic?
Solutions on either side of a semi-permeable membrane have the same solute concentration
What does it mean when solutions on either side of a semi-permeable membrane have the same solute concentration?
Isotonic
What is Hypertonic?
Solution wth higher concentration of solutes relative to that on the other side of the membrane
What is it called when solution with higher concentration of solutes relative to that on the other side of the membrance?
Hypertonic
What is hypotonic?
Solution with lower concentration of solutes relative to that on the other side of the membrane
What is it called when a solution has a lower concentration on one side of the membrance?
Hypotonic
What is a Barophile?
Can survive under extreme pressure and will rupture if exposed to normal atmospheric pressure
What can survive under extreme pressure and will rupture if exposed to normal atmospheric pressure
Barophile
Chlamydomonas nivalis grows on Alaskan glaciers and its photosynthetic pigments give the snow a red crust. This organism is..
Psychophile
What is symbiotic
Organisms live in close nutritional relationships; requred by one or both membranes
What organisms live in close nutritional relationships and are required by one or both membranes?
Symbiotic
What are nonsymbiotic organisms?
Organisms are free-living; relationships not required for survival
What organisms are free living and don't require relationships for survival?
Nonsymbiotic Organisms
Mutualism
Obligatory, dependent; both membranes benefit.
Commensalism
The commensal benefits; other member not harmed
Parasitism
Parasite is dependant and benefits. host is harmed
Symbiotic break downs...
Mutualism, commensalism, parasitism
Synergism
Members cooperate and share nutrients
Antagonism
Some members are inhibited or destroyed by others
Nonsymbiotic break downs...
Antagonism, Synergism
What inhibits the human body that is commensal, parasitic, and synergistic relationships?
Normal Microbial flora
What results when organisms attach to a substrate by some form of extracellular matri that binds them together in complex organized layers?
Biofilms
Quorum Sensing
Communicate and cooperate in the formation and function of biofilms
Biofilms do nothing to help with structure of most natural environments on earth..true or false?
False
What two levels does microbial growth occur?
size and population
Division of bacterial cells occurs mainly through what?
Binary fission (transverse)
What happens during Binary Fission?
Parent cell enlarges, duplicates its chromosome, and forms a central transverse septum dividing the cell into two daughter cells
What is the time required for a complete fission cycle called?
Generation or double timing
What is exponential growth
Each new fission cycle increases the population by a factor of 2
Generation times vary from minutes to days. True or False?
True
What is the equation for calculating the polulation size over time?
Nf=(Ni)2n
What does N(f) mean?
Total number of cells in the population
What does N(i) mean?
the starting number of cells
What does the exponent n means?
denotes generation time
What does 2(n) mean?
The number of cells in that generation
Escherichia coli has a doubling time of 20 min. If there are 5 cells at the beginning of the experiment, how many will there be in 3 hours?
45
What is this called: populations typically display a predictable pattern over time
Growth curve
What phase is concidered the "flat" period of adjustment, enlargement, little growth
Lag Phase
What is the period of Maximum growth called when the growth continues as long as cells have adequate nutrients and a favorable environment?
Exponential Growth phase
What is the phase called where the rate of cells is equal to the rate of cell death
Stationary phase
What causes cell death during the Stationary Phase?
Depleted nutrients and O2, exretion of organic acids and pollutants
What is the phase called that as limiting factors intensify, cells die exponentially
Death phase
What are the 4 phases in the populations growth curve?
Lag phase, exponential growth phase, Stationary Phase, Death phase
What is the most simple method for Analyzing population growth?
Turbidometry
What is the study that shows the degree of cloudiness, turbidity, reflects the relative population size called?
Turbidometry
What does Enumeration of bacteria show?
Viable colony count. Direct cell count-counts all cells present either automated or manual